It might be a good Idea to use Pbf protected Arg as this is a lot quicker to
deprotect than Arg-Mtr. Arg-Mtr can cause deprotection problems especially
when you have several in the sequence. The Pro and subsequent Arg will
probably need extended coupling because of the bulky side chain on the Pro.
-----Original Message-----
From: fperini@unmc.edu [mailto:fperini@unmc.edu]
Sent: 22 August 2000 23:24
To: Recipients of ABRF List
Cc: Recipients of ABRF List
Subject: Re: Pep Syn
I do not think that the synthesis should be such a problem.The residues are
not basic
until deprotected,and having only two adjecent Arg should pose no
problem.The deprotection
may take longer than usual,depending on the protecting group.
Rebecca Ettling <ettlingr@musc.edu>@aecom.yu.edu> on 08/22/2000 12:09:24 PM
Please respond to ettlingr@musc.edu
Sent by: Association of Biomolecular Resource Facilities
<abrf-request@aecom.yu.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
cc:
Subject: Pep Syn
Hello everyone,
Does anyone have any insights as to whether this peptide:
RRPKG RGKRR RENQR PTDCHL
will be a straight forward synthesis or a difficult one ?
I use Fmoc chemistry with HBTU activation. I am worried about the
synthesis of so many basic residues in very close proximity. If anyone
has any helpful tips, I woud appreciate them very much.
As always, thank you in advance for your advice.
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
If I have seen farther than others, it is because I was standing on the
shoulders of giants.--Isaac Newton
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