My apology, additional info concerning the question:
S/E ratio: 20:1
RT for 2.5 h and 5 h and 37*C for 2,5 h
Buffer: 0.2 M ammonium bicarbonate pH 8
Sequence for KKT.........YKNPK: KKTQLQLEHLLLDLQMILNGINNYKNPK
Ting Wong
Biomira Inc.
-----Original Message-----
From: Ting Wong
Sent: Wednesday, August 23, 2000 10:39 AM
To: 'abrf@aecom.yu.edu'
Subject: re: tryptic digest
Hi, everybody:
On analysis of a tryptic map, I have the following fragments:
KT..........YKNPK
KT...........YK
T.............YKNPK
T..............YK
I know there is a lysine before the K in the sequence, so this is
apparently the result of incomplete digestion of the sequence:
KKT............YKNPK
Does anybody have suggestion on how to achieve complete digestion to
have a clean-cut map? Thanks in advance for suggestions.
Ting Wong
Biomira Inc.
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