I am facing a problem and I need some help to solve it. I cleaved a long
peptide with iodosobenzoic acid and I separated the peaks by reversed-phase
HPLC. They were big peaks, many surpassed the scale of 1.0 AUFS. After
drying in SpeedVac, I applied some peaks in mass spectrometer and automated
sequencer. I didn't obtain much sign. Since the peaks went out with a
relatively high concentration of acetonitrile (>50%), they should be enough
hydrophobic. Because that, I suppose they should be stuck to the walls of
the polypropylene microcentrifuge tubes. In order to solubilize them, I
intend to use a 3:1 solution of methanol:chloroform. Then I intend to add
water and acetic acid in order to apply into an electrospray mass
spectrometer. Could anybody tell me if the chloroform interferes in the
electrospray process? Some other suggestion for the material loss?
Thanks in advance.
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Prof. Ricardo B. Cunha
Brazilian Center for Protein Research and Services - CBSP
Laboratory of Protein Chemistry and Biochemistry
Institute of Chemistry
University of Brasilia
Brasilia - DF Brazil
ZIP CODE: 70910-900
Tel: 55-21-61-307-2142
Fax: 55-21-61-272-4548
mailto:rbcunha@unb.br
http://www.geocities.com/Athens/Acropolis/5221
http://www.unb.br/cbsp
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