One thing I see that I'd worry about is the junk that the chloroform will pull off the
plasticware. You may end up with major amounts of background. You might try a blank run
and see what the background from your containers looks like first...I have used chloroform
in esi and usually get something weird either from background or adduction. Have not done
it much however. I got usable results some of the time.
I'd want some protic source for the esi peptide analysis (so maybe dilute into the usual
esi solutions once you recover it). Did you think about tfa or formic as a way to pull
the peptides off the walls? Might be simpler.
Matt Sweeney
mattsweeney@earthlink.net
Mass Spec Consulting
Training/Operations/Consulting/Method Development
LC/MS Pharmacokinetics, Peptides, Proteins, Metabolism,
Maintenance Classes, Specialist in Finnigan Equipment and Software
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