Your problems may be related. There is a board that controls the sample
tranfer. We were getting random 30%-40% failures and after the board was
replaced things recovered well. My impression was that the
reliability/quality ofthe board was questionable. If it is not working
properly it could result in failures and/or carry over to the next
capillary. Otherwise I would check the syringes and lines used in the
sample transfer. I usually use water as the sample loading solution and
failures are very occassional. By failures I mean samples that should have
sequence but show nothing or unusable data.
Martin
>Hello all,
>
>We are experiencing several problems with our 3700. Particularly at the
>moment, we see cross-over of lanes - especially where a capillary has no
>sequence in it (just water), we see 200 of good data taken from another
>capillary. Has anyone else seen anything similar? It is happening to us
>with frightening regularity!
>
>Kind Regards
>
>Andrew
>
>-----Original Message-----
>From: Walding Andrew
>Sent: 24 August 2000 12:43
>To: Recipients of ABRF List
>Subject: Injection on the 3700
>
>
>Has anyone done much optimising of injection voltage and times on the 3700?
>
>What are your conclusions? We would like to optimise ours for plasmid
>sequencing, as we are seeing a very high failure rate of our samples.
>
>Thanks
>
>Andrew
This archive was generated by hypermail 2b29 : Fri Aug 25 2000 - 08:35:04 EDT