Hello
Since DNA has a uniform charge density, separation by ion
exchange gives you a separation that correlates with size. Some
sequences with a bit of structure won't run as expected. You can
get resolution about the same as an agarose gel by using a strong
anion exchanger = small (<2-3 micron) nonporous spherical
packings on HPLC. You'd run a chloride (NaCl) gradient. After
collection all that's needed is desalting. Pebio and Waters have
such columns. Agarose electophoresis would be my first choice.
Use a preparative comb and free the DNA from the agarose by
using freeze/thaw, chaotropic agents like sodium iodide or
perchlorate, agarase digestion etc.
B. Thomas
Brad Thomas
Director, Bioinformatics
SeqWright, Inc.
Voice: 713-528-4363
bthomas@seqwright.com
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