Protein N-terminus

From: Christoph W. Turck (turck@itsa.ucsf.edu)
Date: Fri Aug 25 2000 - 18:04:16 EDT

    We want to determine the N-terminal amino acid sequence of a protein.
Edman degradation did not yield any results, so we suspect a blocked
N-terminus. Are there any methods determining the N-terminus using mass
spectrometry? How likely is it that one finds the N-terminal tryptic
peptide of the protein after an in-gel digest? Or are there any tricks that
one can use to increase one's chances of finding the N-terminal peptide?

    Thanks,

Chris (turck@itsa.ucsf.edu)

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