I have had pretty much the same experience as Jim with the Bio-Rad Protean
IEF Cell; it runs well, and it's easy to program and edit. We went with the
BioRad system a year ago because it was cheaper and the sales reps were much
more accomodating and knowledgable, although, as Jim pointed out, our most
recent rep appears to be a step above what we have had in the past.
I have used Pharmacia's Multiphor for running strips, but not the IPGphor.
By using Pharmacia's reswelling tray (~$200) for the rehydration step
instead of the disposable trays sold by Bio-Rad (which are very convenient
for storage and for equilibration), we have gotten very consistent results
with Bio-Rad's 2-D strips. I would rate them about the same for quality,
although Pharmacia has more variety.
Cristine Charlesworth, Ph.D.
Mayo Protein Core Facility
Mayo Foundation
Tel: 507 284-0912
Fax: 507 284-2053
> ----------
> From: Maria_Parkman@berlex.com
> Sent: Thursday, August 24, 2000 1:03 PM
> To: Recipients of ABRF List
> Subject: 2-D electrophoresis
>
>
>
> Dear Colleques,
>
> Our company is in the process of comparing two systems for 2-D
> electrophoresis. They are the Pharmacia IPGphor and the Bio-Rad Protean
> IEF Cell .
> We are not equipped with any software or densitomers as yet. If you
> could please give us some input on either of these systems and the
> necessary accessories from these companies, we would greatly
> appreciate your help.
>
> Look forward to hearing from you, Thank you very much.
>
> Maria Parkman,
> Berlex Biosciences
>
>
>
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