Good morning, Tevie !
As to your problem of gelatin-containing samples to be prepared for
MALDI-MS:
I think you have to get rid of the gelatine ! Your sample won't crystallise
and, moreover, gelatine (highly X-linked collagen-like proteins) may give
you background signals. You want to analyse proteins/peptides, am I right ?
You can try a simple RP-purification (SepPak (Waters) or a micro-column,
depending on your sample amount). Gelatine will stick to the column whereas
you can elute your peptide/protein sample with a moderate amount of ACN. I
case of very little sample, you can prepare tiny columns in a GelLoader tip
and directly elute with matrix.
If you absolutely want to get around a small-scale purification step, you
could also try a sample prep with nitrocellulose (sandwich method).
You may need to heat you sample solution a bit, since gelatine is soluble
in warm water but becomes viscous when cold.
Most of these sample-prep tricks are summarised in:
Kussmann, Roepstorff et al., J. Mass Spec. 1998, and Kussmann, Roepstorff,
Meth. Mol. Biol. 2000.
Hope this will help. Good luck and best regards,
Martin.
*****************************
Martin Kussmann, PhD
R&D, Protein Analysis /
Mass Spectrometry
CERBIOS-PHARMA S.A.
Via Pian Scairolo 6
CH - 6917 Barbengo (Lugano)
Switzerland
Phone: ++41 - 91 - 985 63 11
Fax: ++41 - 91 - 985 63 25
E-mail: rdbio2@cerbios.ch
-----Original Message-----
From: Tevie mehlman [SMTP:tevie.mehlman@weizmann.ac.il]
Sent: mercoledi, 30. agosto 2000 18:16
To: Recipients of ABRF List
Subject: mass spec-gelatin
Has anybody ever tried to get mass spec data on a sample that contains
Gelatin? We are using a Bruker Reflex III MALDI mass spec. When I use
Synapinic Acid with the sample, it turns yellow and becomes very viscous.
It appears that I am not getting any crystals. Any suggestions?
Tevie Mehlman
Weizmann Institute of Science
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