Re: phosphoproteins and immunodetection

From: Dan Crimmins (crimmins@pathbox.wustl.edu)
Date: Thu Aug 31 2000 - 09:13:47 EDT


At 05:02 PM 8/30/2000 -0300, jaime paba martinez wrote:
>Dear All,
>I am willing to detect some phosphoproteins in 2D gels
>by immunoblotting and i have heard that some of the
>comercial antiphosphotyrosine/serine/theronine
>antibodies are not really specific. I would like to
>know which supplier has a good reliable stock of these
>antisera.
>
>any suggestion will be helpful,
>
>Jaime Paba
>Cell Biology Department
>University of Brasilia
>Brazil
>
>
>
>

Jaime,
        Have a look at the Cell Signaling Technology website.

        www.cellsignal.com

Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu



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