RE: PepSyn:solubilizing and purifying phospho peps

From: neil brew (neil.brew@bbsrc.ac.uk)
Date: Thu Aug 31 2000 - 09:30:36 EDT


Dear All
        I have the following two Phospho-peptides to make;

V S S D G H E Y* I Y* V D P M Q L P Y D S T

K E P E E R P T F E Y* L Q A F L E D Y

Y* = Tyr(PO(OH,OBzl))-OH

Both will be made on a pioneer Fmoc synthesiser. I was going to use
HATU .49M, DIPEA 1M activators, 20% piperidine deblock. 1hr couple for all
A.A's, with Fmoc monitoring and auto extend deblock and couple.
        Anybody have anything to add/suggest that would aid in these
syntheses?



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