Hello
2D gel problems:
My two SDS gels ran at a quarter of the current I'd usually expect. It
took three times as long as usual for the dye front to come to the end
of the gel.
On silver staining one gel, it showed very nicely focussed spots
clustered together in the top third of the gel, and a distorted wavy
brown front at the bottom (about where the dye front eventually got to).
This front is only under where the IEF strip was placed - the MW markers
are nice and clear.
This makes me wonder if it is an equilibration buffer contaminant
problem. The MW markers are perfect.
My question then is this: would oxidised DTT in the equilibration buffer
cause such an effect?
Any advice would be greatly appreciated.
Regards
Di Barraclough
Postharvest Group
HortResearch
Auckland
New Zealand
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