Your first peptide contains an Asp - Gly sequence. You
could develop a problem with dehydration at this step. I
have found that by using FMOC-Asp(O-1-Ada)-OH and
FMOC-(FmocHmb)Gly-OH, both sold by Novabiochem, you can
suppress the formation of aspartamiide.
Good luck,
Jay Gambee
On Thu, 31 Aug 2000 14:30:36 +0100 neil brew
<neil.brew@bbsrc.ac.uk> wrote:
>
> Dear All
> I have the following two Phospho-peptides to make;
>
> V S S D G H E Y* I Y* V D P M Q L P Y D S T
>
> K E P E E R P T F E Y* L Q A F L E D Y
>
> Y* = Tyr(PO(OH,OBzl))-OH
>
> Both will be made on a pioneer Fmoc synthesiser. I was going to use
> HATU .49M, DIPEA 1M activators, 20% piperidine deblock. 1hr couple for all
> A.A's, with Fmoc monitoring and auto extend deblock and couple.
> Anybody have anything to add/suggest that would aid in these
> syntheses?
>
----------------------
Jay Gambee
JEG@shcc.org
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