A customer wants to order a fluorescently labeled peptide. So far I
have found two possible protocols. One adds a beta-alanine or other
spacer onto the N-terminus of the peptide and reacts that with FITC.
The other protocol adds 5-carboxyfluroscein onto the N-terminus of the
peptide.
O.K. so my question is: Is there a rational approach to deciding which
method would be better for my client's peptide ?
Thank you all again for your help !
-- Rebecca P. Ettling
Biotechnology Resource Laboratory
Protein Sequencing and Peptide Synthesis Facility
Medical University of South Carolina
173 Ashley Avenue, Room 733D BSB
Charleston, SC 29403
Tel.: (843) 792-1271
Fax: (843) 792-1264
If I have seen farther than others, it is because I was standing on the
shoulders of giants.--Isaac Newton
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