At 11:40 AM 9/1/2000 -0500, Beverly DaGue wrote:
>Does conversion of Asn-Gly to Asp-Gly readily occur during trypsin
>digestion of proteins in 50 mM ammonium bicarbonate?
>MS/MS data of tryptic digests of a protein of interest to us, show the
>presence of a peptide with both Asn-Gly and Asp-Gly at a particular site
>after 6-8 hours of digestion. However, only the Asp-Gly form of the
>peptide is present after overnight digestions. Any good literature
>references to this phenomenon would be greatly appreciated.
>Thank you.
>Beverly DaGue
>Sr. Research Assistant
>Mass Spectrometry Research Center, 808 MRB1
>Vanderbilt University Medical Center
>Nashville, TN 37232-6400
>
>
>
>
Beverly,
See Deamidation and Isoaspartate Formation in Peptides and Proteins
edited by Dana Ashwad, CRC Press 1995.
Regards,
Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu
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