This reference might help. Unfortunately the author and good friend was
killed in a plane crash earlier this year. This method worked well for
separating a trypsin enzyme with a pI=9.0.
Madden, Mark. Reverse IEF Procedures Reserves Charge Variants of Basic
Proteins. Analytical <http://www.apnet.com/www/journal/ab.htm>
Biochemistry. 229: 203-206 (1995).
Kim Brown
Novo Nordisk Biotech, Inc.
Davis, California
USA
-----Original Message-----
From: Panagiotis Stasinakis [mailto:pstasina@mail.pasteur.gr]
Sent: Friday, August 11, 2000 11:47 AM
To: Recipients of ABRF List
Subject: basic protein: isolation using ief
I have to isolate a protein using ief. I read for reverse ief, because my
protein is too basic. Does anyone has ever use such protocol? Any other
suggestion?
I would like to thank you in advance for any information.
Panagiotis Stasinakis
Phd student
Hellenic Institute Pasteur
Departement of Biochemistry
Vas. Sofias 127
GR 11521, Athens
Greece
pstasina@mail.pasteur.gr <mailto:pstasina@mail.pasteur.gr>
+030 01 6478840
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