Dear Millie:
I have successfully used DMSO (15% in DMF and up to 100%) as
(additional) solvent in case of difficult couplings. Unprotected Met
may oxidize during prolonged couplings.
A useful reference is: C. Hyde , T. Johnson R.C. Sheppard, J. Chem.
Soc. Chem. Commun. 1992, 1573-1575.
I also have a Rainin Symphony but never tried DMSO either. If used
it in a low percentage (15-20%) it should be fine.
Best of luck!
Henriette
>Has anyone using the Symphony Peptide Synthesizer ever used a small amount of
>DMSO in the amino acid bottles to help prevent crystallization of amino acids
>and possible line blockage? Could it possibly also positively effect some
>problematic couplings? Just something I've wondered about for a long time but
>not brave enough to try. Also, how about compatibility with the
>fluidics on the
>instrument.?
>
>Millie McAdams/ HHMI Biopolymer Facility
>919-684-2652
>mcada002@mc.duke.edu
Henriette A. Remmer, Ph.D
Director of Protein Services
Protein Sciences Facility at the Biotechnology Center
University of Illinois
311/315 Noyes Laboratory, Box 62-1
505 S. Mathews Ave.
Urbana,IL 61801
Tel: (217) 333-4695 (Lab) (217) 333-3841 (office)
Fax: (217) 244-1142
homepage: http://www.life.uiuc.edu/biotech/protein_sc.html
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