RE: Rainin Symphony Users

From: Carter, Mark (MCarter@axcellbio.com)
Date: Fri Sep 08 2000 - 11:32:06 EDT

t 15:30 07/09/00 -0400, you wrote:
>
>Has anyone using the Symphony Peptide Synthesizer ever used a small amount
of
>DMSO in the amino acid bottles to help prevent crystallization of amino
acids
>and possible line blockage? Could it possibly also positively effect some
>problematic couplings? Just something I've wondered about for a long time
but
>not brave enough to try. Also, how about compatibility with the fluidics
on the
>instrument.?
>
>Millie McAdams/ HHMI Biopolymer Facility
>919-684-2652
>mcada002@mc.duke.edu
>Millie

For about 4 years of usage, I always used neat DMSO to dissolve all my amino
acids for the Symphony. This gives you about 20-35% DMSO in your coupling
mixture, depending on how your cycle looks and the internal swelling volume
of your resin. Using neat DMSO to dissolve the amino acid reagents never
gave me any trouble at all, but I never tried using solutions more than a
week old. The superior dissolving power of DMSO let me make the AA's (and
biotin!) at more than 200 mM with no problem. And because the Symphony
reacts under a nitrogen blanket, there is no problem with facilitated
oxidation of Met. I recommend you go for it!

Mark 

--
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.
.
.
.
Dr. John Mark Carter
MCarter@AxCellBio.com

AxCell Biosciences Corporation
826 Newtown-Yardley Road, Suite 100
Newtown, PA  18940-1721
office  267.757.1223
lab  267.757.1230
FAX  267.757.1301
.
.
Axcell Biosciences is
a wholly owned subsidiary
of the Cytogen Corporation.
.
.
.
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.
"Ohne Analyse, keine Synthese."
[Without analysis, no synthesis.]
	-Friedrich Engels, Anti-D¸hring, 1878

-----Original Message-----
From: mcada002@mc.duke.edu [mailto:mcada002@mc.duke.edu]
Sent: Thursday, September 07, 2000 3:31 PM
To: Recipients of ABRF List
Subject: Rainin Symphony Users

Has anyone using the Symphony Peptide Synthesizer ever used a small amount
of
DMSO in the amino acid bottles to help prevent crystallization of amino
acids
and possible line blockage?  Could it possibly also positively effect some
problematic couplings?  Just something I've wondered about for a long time
but
not brave enough to try.  Also, how about compatibility with the fluidics on
the
instrument.?

Millie McAdams/ HHMI Biopolymer Facility
919-684-2652
mcada002@mc.duke.edu

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