Bob,
Use the standard 6M guanidinium HCl to extract your protein, purify/buffer
exchange on size exclusion to something that will be tollerated on
immunization. If it's in inclusion bodies, you'll probably have to settle
for ~4M urea in your final buffer to keep it in solution.
Roger
At 01:43 PM 9/13/00 -0400, Bob Lee wrote:
>Hi,
>
>Need a good way to prepare the protein from the inclusion body of the E.
>coli for raising polyclonal antibody. Thanks.
>
>
>Bob
------------------------------------------------------------
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au
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