I cannot tell you anything about the enzymes,because they did not hold any
interest.But,now that you brought up the subject,I would say that if they
are active and specific,and any other protease activity be kept in
check,they may have some use.There are enzymes which clip before aspartic
acid,and,after glutamic acid,respectively,but not at the corresponding
amide.By using such enzymes,additional sites would be
exposed.However,repeated exposure to acid,conditions which are used in
protein sequencing,have shown extensive deamination.Long time ago,I recall
that someone showed that the rate of deamination,in strong acid,is much
faster than than the rate of cleavage at the peptide bond.
Ting Wong <TWong@biomira.com>@aecom.yu.edu> on 09/12/2000 06:04:41 PM
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Subject: glutaminase and asparaginase
To all:
I wonder if anybody has experience on deamidation of Asn and Gln in
peptide or protein using enzymes such as glutaminase or asparaginase?
This is a totally new subject to me, so any idea on the general area of
deamidation of protein (chemical or enzymatic) would also be
appreciated.
Ting Wong, Ph.D.
Analytical development scientist
Biomira Inc.
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