Is this a serious problem?How do you determine that you have
aggregation?Subunits can be help together by a
variety of forces,which will depend on the primary sequence of the
molecule.I had a peptide with a sequence related
to collagen,and,I never got a clean preparation.It is supposed to
aggregate.Heat may help ,or chaotropic
salts(perchlorate,guanidine,etc.),but there is no generalized solution.
tonareli@fbcb.unl.edu.ar@aecom.yu.edu> on 09/14/2000 05:48:40 PM
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Subject: agregation
I have an aggregation problem with a synthetic cyclic peptide of 15
aa. We want to purifie up to 95% by reverse phase-preparative
HPLC. Could someone help me?
Thank you in advance.
Dra.Georgina Tonarelli
Facultad de Bioquimica y Cs. Biologicas
UNL.- Argentina
e-mail: tonareli@fbcb.unl.edu.ar
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