Dirk,
I've routinely used shots (up to 1ml at a time) of 6M guanidine HCl as
restoratives for C18 columns that have seen too much garbage from tissue
extracts. Doesn't harm the column at all.
Roger
At 05:23 PM 9/18/00 +0100, J.betley wrote:
>Dear All,
>
>Does anyone have experience of HPLC of proteins in refold buffers? The
>protein samples contain things such as strong urea and guanidine; we
>wonder what effect this would have on, for example, RP18 HPLC columns. If
>this is a no-no, then are there any suggestions as to alternatives?
>
>Thanks
>
>Dirk
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