Re: pTyr @ C-term?

From: Dr G. Bloomberg (gbloombe@hgmp.mrc.ac.uk)
Date: Mon Sep 18 2000 - 18:43:07 EDT


Works fine. Couple with HBTU or similar. No need for special conditions.

Graham Bloomberg
Peptide Synthesis Facility
Dept. Biochemistry
Medical School
University of Bristol
Bristol BS8 1TD
UK

Tel. +44(0)1179-293205

On Mon, 18 Sep 2000 mcada002@mc.duke.edu wrote:

>
>
> Customer wants a 13-AA peptide with phospho-Tyr on C-terminus with CONH2. Is
> this OK?. I'm sure to do ext'd double couple - but will that do it?
> Composition: 1xCys, 2xGln, 2xPhe, 1xAsn, 1xGlu, 1xAsp, 2xGly, 1xSer, 1xIle,
> 1xTyrp.
>
> If you've had experience with this or a 'gut' feeling, please respond.
>
> Thanks again!...
>
>
> Millie McAdams/ HHMI Biopolymer Facility @ DUCM
> mcada002@mc.duke.edu
> (919) 684-2652
>
>
>



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