Any kind of enzymatic digestion will do better if the protein
unfolded.Neither Lys may be cleaved if they are hidden,or
one or both may be cleaved if the conformation allows.In other words,it is
not the cystine per se,but where it is located.
"Andrews, David" <Andrewsd@biochempharma.com>@aecom.yu.edu> on 09/18/2000
03:03:40 PM
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Subject: trypsin on Lys next to Cys
Does anyone have any experience as to whether trypsin will cleave a lysine
that is adjacent to a cysteine that is participating in a disulfide
bond?....and I mean on either side of the Cys....that is, in the sequence
---K-C-K---, would either lysine be clipped?...
David W. Andrews
David W. Andrews, Ph. D.
Director, Analytical Research & Development
BioChem Pharma Inc.
30 Bearfoot Road
Northborough, MA 01532
508-351-1004 (voice)
508-351-9675 (fax)
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