Do you see anything else in reasonable amounts?Is an amino acid analysis
correct?You may be dealing with + and -
elements.
"Radhika Krishnan" <rkrishna@coh.org>@aecom.yu.edu> on 09/22/2000 11:54:30
AM
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Subject: Masspec: +71 Da
Dear ABRFers,
I cannot identify the origin of a +71Da signal in a sample of a HPLC
purified peptide.
The sequence had the following aa with protected side chains Ser-tBu,
Thr-tBu, Arg-Pbf, Trp-tBoc, and 3 Prolines . Its a 23 mer the rest of
the aa are straight chain aa. I purified the peptide after cleavage
with Cocktail B. I cannot assign this signal to an adduct or
incomplete cleavage product , but the signal has+Na, and +K peaks
also.
Thanks for your help.
Radhika.
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