Hi
It would help if you could post the sequence in full.
Graham Bloomberg
Peptide Synthesis Core Facility
Dept. Biochemistry
Medical School
University of Bristol
Bristol BS8 1TD
UK
On Fri, 22 Sep 2000, Radhika Krishnan wrote:
>
> Dear ABRFers,
> I cannot identify the origin of a +71Da signal in a sample of a HPLC
> purified peptide.
> The sequence had the following aa with protected side chains Ser-tBu,
> Thr-tBu, Arg-Pbf, Trp-tBoc, and 3 Prolines . Its a 23 mer the rest of
> the aa are straight chain aa. I purified the peptide after cleavage
> with Cocktail B. I cannot assign this signal to an adduct or
> incomplete cleavage product , but the signal has+Na, and +K peaks
> also.
> Thanks for your help.
> Radhika.
>
>
This archive was generated by hypermail 2b29 : Fri Oct 06 2000 - 12:35:07 EDT