I'm having a problem posting my messages to the recipients of ABRF.
What's happening?
Below, is my message.
To all members,
I wish to place the TEV cleavage site between two proteins. I will not
be using a His-tag. The protocol I have is for the TAP tag which I don't
need. However, this protocol gives spacer regions prior to and after the
TEV cleavage site. I need to know whether these spacer regions are
required for my application.
Any suggestions?
Sue Sroka
Samuel Lunenfeld Research Institute
Toronto, Ontario
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