Hi Melanie,
We have a HP G1000A Edman sequencer and have been using some ABI reagents on it
for a while. As far as I can tell the performance has not suffered. I use ABI
ethyl acetate (the 450 mL bottle) and PITC/heptane which I dilute to 3% with
heptane also from ABI. TFA (R3) I still get from Agilent and dilute it with MQ
water for R4. I use HPLC grade methanol (L2) and acetonitrile (L1). S4 is HPLC
grade acetonitrile diluted with MQ water. R2 and R2A I get from Agilent.
Our throughput here hasn't been very high over the last year or so and I haven't
bought many reagents. I've ordered R2 and R2A today from Agilent, the first since
the supply change I believe, so it will be interesting to see what the quality is
and how quickly they are delivered.
Melanie Madanat wrote:
> Our lab has a (now obsolete) Agilent 241 instrument which is used exclusively
> for N-terminal sequencing.
> Up until early this year this instrument has generally been robust. However,
> since Agilent has contracted its manufacturing of reagents to Aldrich, I have
> been faced with reagent shortages and "bad" reagents.
>
> The issue of reagent shortages/back orders has just gotten worse over time. I
> have to "track down" the products with the ordering dept. at Agilent and often
> find out that the item has been further back-ordered.
>
> The issue of "bad" reagents has not been addressed properly by Agilent. I
> strongly believe that every Agilent sequencer user should be notified as soon
> as a defective product is identified. Instead we end up calling our service
> engineer to work on an instrument that may have no hardware problem, but is
> "acting up" due to reagent issues.
>
> So, my question to Agilent Sequencer users, where are you currently getting
> your reagents to run the instrument?
> ABI supplies R3, R4 and S2A. Has anyone used this and found them to be
> adequate for the Agilent instrument?
> What about the remaining reagents that ABI does not supply?
>
> Your responses would be greatly appreciated.
>
> Melanie Madanat
> Bayer Corp.
-- Jelle LahnsteinNucleic Acid and Protein Chemistry Unit, Department of Plant Science Waite Campus, The University of Adelaide, Urrbrae, South Australia 5064 Tel: +61 8 83037260 Fax: +61 8 83037130 http://www.waite.adelaide.edu.au/NAPCU/
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