Dear Colleagues:
There is the occasional request on this site for identification of a
laboratory service that will determine the enantiomeric content of a peptide.
A laboratory in Australia (jelle.lahnstein@adelaide.edu.au), responded
(2000-08-17) that they provide such service.
I have just returned from the European Peptide Symposium where I learned
that there is also a company in Germany that provides such service, and I
thought it might be of interest to someone.
It is C.A.T. Gmbh & Co, (Dr. Jurgen Gerhardt), Chromatographie und
Analysentechnik KG, Heerweg 10, D-7400 Tubingen 5, Tel. 7071 78323, Fax
7071 75163. The cost is 470 DM for the first amino acid, and 110 DM each
for the next five amino acids examined. This includes hydrolysis of the
peptide. The analysis can be done on <1 mg of compound or on an eluted
HPLC peak. The method they use involves a long established procedure that
has been refined involving hydrolysis in DCl that allows distinguishing by
MS between the amount of other isomer present in the peptide from that
which is generated during the hydrolysis. The enantiomers are separated by
GLC as the CF3CO-Xaa-OPr on a Chirasil-Val column. They also do simple
amino acid analysis by the same method, by adding before hydrolysis the
tagged D-isomers that serve as internal standards. The service issued
from the developers of the method that goes back to the late 1970's
(Frank, Bayer, Nicholson, Gerhardt,..)
Disclaimer: I have no association of any kind with this company. I
was just intrigued to learn that the method has finally been fine-tuned
enough, with the help of advances in mass spectrometry, to be of practical
value.
Regards. NLB.
N. Leo Benoiton Professor Emeritus of Biochemistry
Faculty of Medicine
University of Ottawa, Tel: 613 562 5800 ext. 8216
451 Smyth Road, Fax: 613 562 5440
Ottawa, Ontario, Canada K1H 8M5 eMail: benoiton@uottawa.ca
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