Bryan,
The two different batches of Jerry's peptides might contain diffent
amounts of scavengers or for that matter (shudder the thought) protecting
groups still attached to the peptides that might affect their 220 and 280
absorbance values--I still vote for Lisa's AAA.
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97403
On Wed, 11 Oct 2000, Smith, Bryan (SLH) wrote:
> Jerry,
> As Lisa Bibbs wrote, AAA would be good since it would also confirm the
> quality of your different batches. On the assumption that they are the same,
> though, why not measure their Abs 220 or 280 nm and adjust their
> concentrations accordingly? In effect, you will have already done that
> measurement during HPLC.
> Bryan Smith
>
> -----Original Message-----
> From: Jerry Kozlowski [mailto:jerrykii@voyager.net]
> Sent: Tuesday, October 10, 2000 11:10 PM
> To: Recipients of ABRF List
> Subject: response factors of peptides
>
>
> Can anyone offer some explanation(s) as to why peptides, supplied from two
> different sources, would have different response factors (area/conc.) when
> chromatographed on the HPLC?
>
>
>
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