Emily,
I used NTCB for cleaving proteins at cysteines, and the method worked
reasonably well. It's been a while, but as, as I recall, one thing to
look for is elimination at cysteins resulting in dehydroalanine
formation. Here's a reference:
I.A. Papayannopoulos and K. Biemann, "Amino acid sequence of a protease
inhibitor isolated from Sarcophaga bullata determined by mass
spectrometry", Protein Science, 1 (1992), 278-288.
Regards,
Ioannis Papayannopoulos
AstraZeneca R&D Boston
At 09:51 PM 10/11/00 -0400, Emily Boja wrote:
>Hi,
>Has anyone used NTCB cleavage for proteins? Any success and tips?
Any
>info will be appreciated. Emily
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