If the peptide is still resin-bound,the entire procedure used in an
automatic sequencer should be used.20% piperidine
in N-methyl pyrrolidone(NMP) can be used,followed by washings with your
solvent of choice.I always end with methanol.
I have used DMF solutions,and triethylamine,in a pinch,as cleaving
bases.Acetylation should be done on the washed resin,using a 2:1 eq. ratio
of acetic anhydride:dimethylaminopyridine.Excess reagent will have to be
removed as
above.I do not any feelings for the amounts which you plan to use,but this
should be a general procedure.
Michael Moore <mmoore@uclink4.berkeley.edu>@aecom.yu.edu> on 10/11/2000
07:41:59 PM
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Subject: resin bound peptide
A student brought a resin bound peptide that they want cleaved and
acetylated.
They're not sure if the final fmoc group was removed. Can someone suggest
manual procedure for removing final fmoc and acetylation prior to cleavage.
Thanks in advance
Michael
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