Jun -
I have an example where cation-exchange and anion-exchange HPLC in sequence
were used with ascites fluid containing 0.6% MAb. After the two runs, the
MAb was 96% pure. Give me your FAX number and I'll send you a chromatogram.
Regards,
Andy Alpert
PolyLC Inc.
9151 Rumsey Road, ste. 180
Columbia, MD 21045
tel: (410) 992-5400 FAX: (410) 730-8340
***************************************************
<< Subj: The column for MAb Purification
Date: 10/17/2000 3:23:26 AM Eastern Daylight Time
From: luojun@er6s1.eng.ohio-state.edu (Jun LUO)
Sender: abrf-request@aecom.yu.edu (Association of Biomolecular Resource
Facilities)
To: abrf@aecom.yu.edu (Recipients of ABRF List)
Hello,
Hybridoma cell culture was used to produce MAb. Also the serum was
added.
I am looking for a good method to separation and purification the MAb.
Could you give any suggestion and information?
Thanks.
Jun LUO
The Ohio State University
luo.32@osu.edu
>>
This archive was generated by hypermail 2b29 : Mon Oct 23 2000 - 10:23:17 EDT