Michel,
How large is your protein? C18 columns, though not sizing
columns per se, do have an upper limit for the size of protein that you
can pass through them--proteins that are too large are just stuck on the
front end of the column and never make it out. The actual
maximum molecular weight probably depends on the dimensions of the now, not
native, who knows how it is folded, protein as it is eluted in the column
mobile phases.
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97403
On Thu, 19 Oct 2000 Michel.Chevalier@aventis.com wrote:
> Dear colleagues,
> Can someone tell me what is the real chance to break a dissulfide bridge
> during LC-MS analysis ?
> This question arise in my lab since we find only monomers of a protein by
> LC-MS and other colleagues find monomers and dimers (70/30) by SDS-PAGE.
> Thank you for your comments.
> Sincerely
>
> Michel Chevalier
>
> Aventis Pasteur
> 1541 Ave M. Merieux
> 69280 Marcy l'Čtoile, France
> Tel : (33)4 37 37 36 85
> Fax : (33)4 37 37 31 86
>
>
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