At 08:31 19.10.00 -0400, you wrote:
>Andrew.Walding@astrazeneca.com wrote:
>>
>> Hi all,
>>
>> Does anyone have a protocol for precipitating 384 well sequencing plates?
>> Our 3700 is finally working after 5 weeks down, so we have a backlog of
>> stuff to fast-track through!
>
>
>I can't help you with this, but a related question for the list participants:
>
>Has anyone tried the Millipore untrafiltration product that purports to
>clean up sequencing reactions in 384-well plates? It's called
MultiScreen384-SEQ,
>and basically appears to be a membrane filter in a 384-well frame, intended
>to be used on a vacuum manifold. Cost is supposed to be around $0.15 per
well.
>
>Bob Lyons
>University of Michigan
Dear Bob,
we are currently cleaning up our our sequencing reactions in the
MultiScreen96-plates. From your description, I am not sure if they are
using the same principle because they contain a filtration membrane and are
filled with sepharose G-50. The workup can be carried out by vacuum suction
or centrifugation alternatively. If they are related to each other, I can
tell you, that they work fine (at least for us). Sample cleanup is much
faster than with precipitation, requires less manual steps and the product
quality is good. We almost never see dye blobs from remaining terminators
in our sequencing reactions. We pay about $35 per plate, but they can be
used at least twice (you only have to exchange the sepharose), so the price
will drop down to below $0.10 per reaction plus the sepharose.
Yours sincerely,
Marcus
********************************************************
Dr. Marcus Macht
Universit”t K–ln
Zentrum f¸r molekulare Medizin
Zentrales Servicelabor
Joseph-Stelzmann-Str. 52
50931 Koeln
Tel.: +49 221 478-6995
Fax: +49 221 478-6977
e-mail: Marcus.Macht@uni-koeln.de
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