ABRFers
While Deb is the last person I would want to disagree with since she
has been such a good supporter of my products, I feel I need to
clarify the relationship between RPC and sizing of proteins. The
issue is probably a chemical one.
We have been supporting a client who is using a 300A, C-4, 5um
particle size column to chromatograph virus particles. The
implication here is that there is not a physical size limit for the
columns, but probably a hydrophobic limit as she correctly states.
Generally, the frit porosity is the physical barrier to consider.
Best regards,
Amos Heckendorf
The Nest Group
>Michel,
> How large is your protein? C18 columns, though not sizing
>columns per se, do have an upper limit for the size of protein that you
>can pass through them--proteins that are too large are just stuck on the
>front end of the column and never make it out. The actual
>maximum molecular weight probably depends on the dimensions of the now, not
>native, who knows how it is folded, protein as it is eluted in the column
>mobile phases.
>
>
>Deb McMillen
>Institute of Molecular Biology
>University of Oregon
>Eugene OR 97403
>
>On Thu, 19 Oct 2000 Michel.Chevalier@aventis.com wrote:
>
>> Dear colleagues,
>> Can someone tell me what is the real chance to break a dissulfide bridge
>> during LC-MS analysis ?
>> This question arise in my lab since we find only monomers of a protein by
>> LC-MS and other colleagues find monomers and dimers (70/30) by SDS-PAGE.
>> Thank you for your comments.
>> Sincerely
>>
>> Michel Chevalier
>>
>> Aventis Pasteur
>> 1541 Ave M. Merieux
> > 69280 Marcy l'»toile, France
> > Tel : (33)4 37 37 36 85
> > Fax : (33)4 37 37 31 86
> >
> >
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