Hi Mike,
yes I too had this problem a while ago with low levels of ARG even with a new batch of PTH standard. The procedure in the Procise manual suggests using volumetrics (glass) to accurately measure your PTH standard. The loss of ARG I found was due to adsorption onto the glass. I just spiked standard directly into the polypropylene bottle in the R5 position and that solved the problem. Are you using a glass bottle in the R5 position?.
hope this helps
Dougie
University of Dundee
Scotland
United Kingdom
----- Original Message -----
From: Michael Mahar
To: Recipients of ABRF List
Cc: tt34@cornell.edu
Sent: Friday, October 20, 2000 2:59 PM
Subject: ProtSeq
Hello,
I run a PE Procise sequencer. I just started to get a weird problem. My Arg peak in my standard is getting smaller and smaller, each time I run it. I have made up new standard and the same thing just starts all over again. Has anyone run into this problem before? If so how did you fix it?
Thanks
Mike
______________________________________________
Michael J. Mahar
DNA Synthesis / Peptide Sequencing Facility
Cornell University
(607) 254-4848
--Where ever you go there you are--
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