Re: DNA seq: loading 96 well 377?

From: Linda Ballard (linda.ballard@hmbg.utah.edu)
Date: Mon Oct 23 2000 - 18:42:26 EDT


on 9/18/00 11:31 AM, Martin Pentony at mpento@cmgm.stanford.edu wrote:

> Has anyone any preferences or recommendations on a syringe/pipette for
> loading the 96 well 377. We are currently using a hamilton
> multi-channel on a 64 lane 373 and I am quite happy with it. Has anyone
> tried the adjustable hamilton for the 96 well setup? Thanks
> Martin
>
>
Martin,

There is also the Prism Pet sold by several vendors. Its spacing is
consistent with the ABI 96-well comb. You use the .4mm stepped plates and a
96-well expandable tray. That may seem like a lot of trouble, but after
trying other 96-well loading methods, this is the one my technicians prefer.
They say it makes loading the 96-well comb just as easy as the 64-lane comb.

Linda

Linda Wood Ballard
Genomics Core Facility
University of Utah
50 N. Medical Dr. 4A430A SOM
Salt Lake City, UT 84132

801-581-3875
www.cores.utah.edu/genomics
  



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