pegylation

From: Maria_Parkman@BERLEX.COM
Date: Wed Oct 25 2000 - 12:10:27 EDT

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Hi,
We are trying to depegylate a ~46K protein which was previously pegylated
with Peg 5000.
The purpose of this study is to map the pegylation sites. Our protein is
pegylated with succinimidyl succinate method and we are trying to brake the
ester bond leaving the amide bond intact.
I am looking for suggestions for complete depegylation conditions and free
Peg removal.

Thank you.
Maria_Parkman@berlex.com

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