Dear ABRF Members,
I run an ABI 377 DNA Sequencer and use the software from ABI. A sample
submitted to our facility sequenced well to ~400 bases and then the signal
was completely lost for ~50 bases. ( A series of N's in the
electropherogram). The signal was then restored for the remainder of the
sequence. Does anyone know why this would occur?
Thanks in advance.
Rhonda Polchak
Sequencing Core
Heska Corporation
Fort Collins, CO 80525
polchar2@heska.com
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