Rhonda-
I have seen this when there is a string of bases- my bet is "T". Often the T
is weak, and in a string they can just drop out. I have tried to recover it
by clipping the bases before and after the affected spot. Unfortunately, I
usually don't get good results.
Sheryl Christofferson
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Oklahoma Medical Research Foundation
DNA Sequencing Facility
Oklahoma City, Oklahoma
(405) 271-7393 ext. 34565
www.omrf.ouhsc.edu/OMRF/Core/DNASEQ/
sherylc@omrf.ouhsc.edu
"Polchak, Rhonda" wrote:
> Dear ABRF Members,
> I run an ABI 377 DNA Sequencer and use the software from ABI. A sample
> submitted to our facility sequenced well to ~400 bases and then the signal
> was completely lost for ~50 bases. ( A series of N's in the
> electropherogram). The signal was then restored for the remainder of the
> sequence. Does anyone know why this would occur?
> Thanks in advance.
>
> Rhonda Polchak
> Sequencing Core
> Heska Corporation
> Fort Collins, CO 80525
> polchar2@heska.com
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