Dear ABRF members:
You should have received the following study announcement by mail. If you
did not receive this and would like the study results sheet sent to you, or
if you have any questions, please contact me at 410-706-8553 or
nambulos@umaryland.edu.
SAMPLES ARE DUE DECEMBER 1, 2000!!
The ABRF Peptide Synthesis Research Group would like to invite member
laboratories to participate in the 2001 study. This year¼s study is
designed to provide an updated examination of improvements to peptide
synthesis chemistry, in particular, those changes that facilitate synthesis
of difficult sequences. Peptide synthesis labs are continually challenged
with peptides that are often challenging at best. Synthesis of such
difficult peptides can yield little or no desired product. Even if the
desired product is present, purification can be just as challenging.
Development of improved methodologies, synthesis reagents, and amino acid
analogs may potentially offer new solutions for the old problem of difficult
sequences. The PSRG would like to compare synthesis schemes and
purification strategies that different member laboratories employ to improve
the quality and separation of the crude peptide. Analysis performed by the
PSRG will include analytical HPLC, AAA, MALDI-MS, ESI-MS.
The PSRG would like participating labs to synthesize the following peptide:
H-QTSSGTTSWVTSRRDGAGAGP-OH
The peptide was designed to encompass a number of side reactions and
difficult stretches. For example, the C-terminal Gly-Pro, if synthesized
using a standard Wang resin will likely result in diketopiperazine
formation, resulting in little to no yield. A number of alternative resins
have been developed which can be used which prevent diketopiperazine
formation.
In addition to comparing synthesis strategies with the crude product, the
PSRG would also like to determine success rates in purifying peptides from a
heterogeneous mixture. To accomplish this, we would like participating labs
to also purify a portion of the peptide that will then be sent to the PSRG
for analysis along with the crude material. We ask you to also note the
amount of crude peptide you began purification with and the final yield to
obtain a percentage of recovered, purified peptide.
Please send five 1mg aliquots of crude and five 1mg aliquots of purified
material, and the materials and methods form to:
Ms. Christy Sasiela
University of Maryland School of Pharmacy
20 North Pine Street, Room 500
Baltimore, Maryland 21201
410-706-2961
This archive was generated by hypermail 2b29 : Thu Nov 09 2000 - 09:18:08 EST