Maria: It has been awhile, but we "depegylated" a protein in 1993. See
M. M. Vestling, C. M. Murphy, D. A. Keller, C. Fenselau, J. Dedinas,
D. L. Ladd, M. A. Olsen, Drug Metabolism and Disposition 21: 911-917
(1993) [A strategy for characterization of polyethylene
glycol-derivatized proteins. A mass spectrometric analysis of the
attachment sites in polyethylene glycol-derivatized superoxide
dismutase]
Also good reading:
J. Bullock, S. K. Chowdhury, D. Johnston, Anal. Chem.68: 3258-3264 (1996)
[Characterization of poly(ethylene glycol)-modified superoxide
dismutase: comparison of capillary electrophoresis and
matrix-assisted laser desorption/ionization mass spectrometry]
Hope this helps.
MMV
Martha M. Vestling, Ph.D.
Mass Spectrometry Laboratory
Chemistry Department
University of Wisconsin - Madison
1101 University Avenue
Madison, WI 53706
vestling@chem.wisc.edu
<Hi,
<We are trying to depegylate a ~46K protein which was previously pegylated
<with Peg 5000.
< The purpose of this study is to map the pegylation sites. Our protein is
<pegylated with succinimidyl succinate method and we are trying to brake the
<ester bond leaving the amide bond intact.
<I am looking for suggestions for complete depegylation conditions and free
<Peg removal.
<Thank you.
<Maria_Parkman@berlex.com
This archive was generated by hypermail 2b29 : Thu Nov 09 2000 - 09:18:08 EST