Maria,
The bridge between these PEG derivatives and protein amine groups includes an
ester bond. These ester bonds are hydrolyzable in mild aqueous alkali (pH 8 -
9.5). Monitoring reaction progress should be possible using standard RP-HPLC.
Once complete, you will be left with succinate derivatives of any amines that
had originally been tagged with the PEG. Separation of the liberated PEG from
protein should be readily accomplished using dialysis (20 kd cutoff should work
fine), RP-HPLC or ion exchange.
Hope this helps,
Russ Lehrman
<Hi,
<We are trying to depegylate a ~46K protein which was previously pegylated
<with Peg 5000.
< The purpose of this study is to map the pegylation sites. Our protein is
<pegylated with succinimidyl succinate method and we are trying to brake the
<ester bond leaving the amide bond intact.
<I am looking for suggestions for complete depegylation conditions and free
<Peg removal.
<Thank you.
<Maria_Parkman@berlex.com
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