Linda,
I'm wondering what "scientific community" the EB is thinking about. I think
your study is well suited to the ABRF scientific community that needs this
yearly yardstick of the protein sequencing sample to see how improvements in
technology are impacting on protein analysis. A mixture that includes Edman
sequencable proteins as well as more difficult lower percentage proteins I
would think would be ideal for 2000-1 when the instrumentation for analysis
are so varied.
Are the yearly samples that research groups send out derivative, general and
not normally suitable for publication? Probably. Is it worth doing so that
ABRF facilities can see how the technology is changing and seeing whether
they are keeping up? I say yes. Is the data more valuable if it takes nine
months or more to see the light of day because it is being peer reviewed? I
would say it is less valuable because in nine months the techniques and
instrumentation have changed.
Is there instrumentation that allows the internal sequence to be perceived
from a few femtomoles? Yes. Should the protein analysis group send out a
femtomole sample to test these instruments only? That would generate a ton
of useful data.... Not.
If you have the sample laying around, could you send me one?
And since I've just donned my asbestos suit I feel safe in throwing in my
two cents again about making the abrf discussion group password protected
and for ABRF members only. Let's think how useful those non-paying lurkers
are. Are we doing it to get a few more membership dollars and miss out on
their expertise? Are we going to force people who don't really fit the ABRF
mold (whatever that is) to become ABRFers just so they can listen in on the
discussion group? Are we doing it to get rid of junk mailers? Are these
valid reasons? OK, I'm getting carried away with the questions.
Let's have a discussion.
Paul, (I know sequencable is not a word), Morrison
____________________________________
Paul Morrison JFB216 paul_morrison@dfci.harvard.edu
Molecular Biology Core Facilities
Dana-Farber Cancer Institute http://mbcf.dfci.harvard.edu
44 Binney Street 617-632-3082
Boston, MA 02115 fax 632-4814
____________________________________
-----Original Message-----
From: Linda Siconolfi-Baez
To: Recipients of ABRF List
Sent: 10/30/00 11:15 AM
Subject: Re: Prot Seq Res Group
Rebecca,
Unfortunately, there will not be a protein sequencing study this year.
In the throes of the Executive Boards process of rethinking its proteomics
strategy,
and the change of the PSRG (protein sequencing research group) to the PARG
(protein
analysis) the study designed by our group was not approved by the board.
Their
criticisms of our study (which would have encouraged participants to
identify as many
components as possible from a lyophilized test sample consisting of a
complex mixture
of intact proteins, the components of variable abundancy, with two proteins
more
abundant relative to the others and identifiable by EDMAN) included that
they
"did not feel that it would lead to data suitable for publication as the
subject was
too general, did not ask sufficiently well defined questions and would not
return
data that would have any statistical significance or impact on the
scientific
community."
I also regret the EB's decision to dissolve our group at ABRF'01 (at
which point our efforts thus far will have served for naught) as part of an
ongoing
Research Group restructuring exercise.
Linda
(PARG, co-chair)
Linda Siconolfi-Baez
Laboratory for Macromolecular Analysis
Albert Einstein College of Medicine
Ullman 405
1300 Morris Park Avenue
Bronx, N.Y.10461
718-430-2864
lsiconol@aecom.yu.edu
----------
>From: Rebecca Ettling <ettlingr@musc.edu>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: Prot Seq Res Group
>Date: Thu, Oct 26, 2000, 9:51 AM
>
>Will there be a Protein sequencing study this year ? I don't see it
>listed.
>
>-- Rebecca P. Ettling
>Biotechnology Resource Laboratory
>Protein Sequencing and Peptide Synthesis Facility
>Medical University of South Carolina
>173 Ashley Avenue, Room 733D BSB
>Charleston, SC 29403
>Tel.: (843) 792-1271
>Fax: (843) 792-1264
>
>If I have seen farther than others, it is because I was standing on the
>shoulders of giants.--Isaac Newton
>
>
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