Hi Bob
I have used with some but, but not total success, a cibacron blue - based
method to try and reduce the amount of serum albumin smearing in 2D gel
images. Using cibacron blue from Sigma (Fast flow cibacron blue 3GA linked
to 6% agarose: prod# C-8321) at slurry : human serum ratios of between 3:1
and 6:1 there is definite improvement in image quality and protein spot
resolution: additional proteins are visualised in the treated sample. The
down side is that a number of proteins disappear and albumin is by no means
completely removed. We now intend to have a look specifically at what in
addition to HSA is removed. The tech bulletin on Affi-Gel blue from
BioRad ( EG Bulletin 1107) has a reasonable list of proteins that are
bound by the resin.
Two other refs of interest:
1) Use of ProtoClear to remove albumin: Elelctrophoresis 1999, 20, 854 -
859. However apparently this never got to market and we were unable to
source it.The paper also makes ref to a kit: ESA, St Ives UK
2) Isolation of albumin from human plasma: Biochem J. 1976, 157, 301 - 306
Derek Van Dyk
Australian Proteome Analysis Facility
Building F7B
Macquarie University
NSW 2109
Australia
http://www.proteome.org.au/
Ph: 61-2-9850 6205
Fax 61-2-9850 6200
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