Tryptic digestion and Maldi-Tof analysis of glutaraldehyde-fixed and silver stained protein spots

From: Nijat Imin (Imin@rsbs.anu.edu.au)
Date: Thu Nov 02 2000 - 03:47:13 EST

Hi all,

Has anyone tried tryptic digesting and Maldi-Tof analysing of
glutaraldehyde-fixed plus silver stained protein spots? I am sure it's
possible to digest proteins even glutaraldehyde covalently binds to the
proteins through Schiff base formation with amino groups. But, does it add
certain masses to the peptides that can be detected by Maldi-Tof? Thank
you.

Nijat IMIN

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