Re: Large MW standards

From: piero pucci (pucci@unina.it)
Date: Tue Nov 07 2000 - 02:17:07 EST


Dear Michael

BSA is not that bad for ESMS analysis. However a better standard in the MW
range that you are analysing is carbonic anhydrase (MW abot 29kDa, you
should find out the exact mass) that is commercially available.
Ciao Piero

At 14.07 06/11/00 -0500, you wrote:
>Dear ABRF'ers-
>
>I have an ESI-LC/MS system and would like to run large proteins (>30kD) on
a C4 column. The problem is that the only standard I have is BSA and I'm
told this is not a well behaved mass spec sample for ESI/MS. Does anyone
know of a good mass spec standard in this range? Many thanks in advance.
>
>Michael Burgess
>Whitehead Institute
>Cambridge, MA
>burgess@wi.mit.edu
>
>
Prof. Piero Pucci
Dipartimento di Chimica Organica e Biochimica
Complesso Universitario Monte S. Angelo
via Cinthia - 80134 Napoli
Tel.: 081-674318 - Fax: 081-674313
e
Centro Internazionale Servizi di Spettrometria di Massa
via Pansini 5 - 80131 Napoli
Tel.: 081-5465136 - Fax: 081-5465484



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