Hi Michael,
the right choice of your standard depends on what you want to do with your
sample. Is it for quantitation? Then the best standard will be a
isotopically labeled "sample" or something chemically very close to your
sample due to the same response in the mass spec. Or is it for m/z
calibration? In this case you are free to choose a peptide or protein
standard for HPLC (Sigma) which covers your m/z (!) range.
Best regards, Markus
Dipl.- Chem. Markus Kellmann
R&D Mass Spectrometry
BioVisioN GmbH & Ko KG
Feodor-Lynen-Str. 5
D-30625 Hannover
Germany
Tel.: +49 511 538896 55
Fax.: +49 511 538896 66
e-mail: m.kellmann@biovision.de
> http://www.biovision.de/
>
> The information contained in this email is intended for the personal use
> of the addressee only. It may also be privileged information. If you are
> not the intended recipient then you are hereby notified that you have
> received this document in error and that any review, distribution or
> copying of this document is strictly prohibited. If you have received this
> communication in error, please notify BioVisioN immediately on (+49) (511)
> 538896-0, or email info@biovision.de
>
> -----Urspržngliche Nachricht-----
> Von: Michael Burgess [SMTP:burgess@wi.mit.edu]
> Gesendet am: Montag, 6. November 2000 20:08
> An: Recipients of ABRF List
> Betreff: Large MW standards
>
> Dear ABRF'ers-
>
> I have an ESI-LC/MS system and would like to run large proteins (>30kD) on
> a C4 column. The problem is that the only standard I have is BSA and I'm
> told this is not a well behaved mass spec sample for ESI/MS. Does anyone
> know of a good mass spec standard in this range? Many thanks in advance.
>
> Michael Burgess
> Whitehead Institute
> Cambridge, MA
> burgess@wi.mit.edu
This archive was generated by hypermail 2b29 : Thu Nov 09 2000 - 09:18:08 EST