Michael,
Lysozyme, horse heart myoglobin, and cytochrome C all work well by +ESI.
The charge state of these species can be influenced by the pH to provide
multiply charged peaks in the m/z range that you desire.
I recommend the following review article. The authors provide an
informative review of the effect of solution conditions on ESI spectra.
They also provide a very extensive list of proteins/peptides with Mr, ESI/MS
info, and associated references.
Proteins in the 30kDa range include:
proteinase K 28079
Carbonic anhydrase 29157
DNAase (recomb., deglycosylated) 29255
Cathepsin B 29077-28762
Thioesterase II 29230-31000
(depending on source and acetylation)
Creatine amidinohydrolase 33776
Thermolysin 34334
glyceraldehyde-3-phosphate dehydrogenase 35700
lactate dehydrogenase 36500
alcohol dehydrogenase 36748
etc....all the way up to 250 KDa
R.D. Smith, J.A. Loo, R.R.O. Loo M. Busman, H.R. Udseth 1991 "Principles and
practice of electrospray ionization-mass spectrometry for large polypeptides
and proteins," Mass Spectrometry Reviews 10:359-451.
Best regards,
Lloyd W. Sumner, Ph.D.
Head, Biological Mass Spectrometry
The Samuel Roberts Noble Foundation
PO Box 2180
2510 Sam Noble Parkway
Ardmore, OK 73402
Voice 580-221-7392
Fax 580-221-7380
Email lwsumner@Noble.Org
-----Original Message-----
From: Michael Burgess [mailto:burgess@wi.mit.edu]
Sent: Monday, November 06, 2000 1:08 PM
To: Recipients of ABRF List
Subject: Large MW standards
Dear ABRF'ers-
I have an ESI-LC/MS system and would like to run large proteins (>30kD) on a
C4 column. The problem is that the only standard I have is BSA and I'm told
this is not a well behaved mass spec sample for ESI/MS. Does anyone know of
a good mass spec standard in this range? Many thanks in advance.
Michael Burgess
Whitehead Institute
Cambridge, MA
burgess@wi.mit.edu
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